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KMID : 0380020100250020123
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Korean Journal of Biotechnology and Bioengineering
2010 Volume.25 No. 2 p.123 ~ p.129
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Antifungal Activity of Bacillus sp. BCNU 2002 against the Human Pathogens
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Choi Hye-Jung
Ahn Cheol-Soo
Jeong Yong-Kee
Kim Dong-Wan
Joo Woo-Hong
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Abstract
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An endospore-forming, rod-shaped bacterium was isolated from forest soil samples collected at the Taebaek mountain of Gangwon province, Korea, and taxonomically characterized by physiological, biochemical and phylogenetic methods. Its 16S rRNA sequences showed the maximum similarity of 97% with B. amyloliquefaciens. In addition, the isolate BCNU 2002 was determined to have the ability to produce enzymes such as amylase, protease, gelatinase and catalase. The in vitro antifungal activity of Bacillus sp. BCNU 2002 was also examined against human pathogenic fungi such as Aspergillus niger, Candida albicans, Epidermophyton floccosum, Saccharomyces cerevisiae, Trichophyton mentagrophytes and Trichophyton rubrum. A maximum production level of antifungal substances of Bacillus sp. BCNU 2002 was achieved under aerobic incubation at 28℃ for 7 days in LB broth. BCNU 2002 showed strong antifungal activities against T. mentagrophytes and T. rubrum with the range of percentage inhibition from 56.25 to 63.23%. It was also confirmed that ethylacetate extract of cultured broth showed a strong antifungal activity against A. niger, C. albicans, S. cerevisiae and T. rubrum by agar diffusion method. The peptide fraction also exhibited broad antifungal spectrum against various pathogenic fungi. The minimum inhibitory concentration values for active extracts ranged between 125 μg/mL and 1000 μg/mL.
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KEYWORD
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Antifungal activity, Bacillus sp. BCNU 2002, human pathogenic fungi, minimum inhibitory concentration
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